Electron Microscopy (EM) Core Facility


The Electron Microscopy (EM) Core Facility facilitates intramural NINDS investigators with the opportunity to use electron microscopic techniques in their research programs. The staff provide assistance in all aspects of electron microscopy including project planning, specimen preparation and training.

Core Staff

Susan J. H. Tao Cheng, Ph.D., Core Director
Phone: 301-496-0579
E-mail: chengs@ninds.nih.gov

Sandra Moreira, Biologist    

Scope of the Core Facility

The Facility is dedicated to providing the scientific community within NINDS equipment for transmission electron microscopy . The Facility also offers expertise in the use and application of microscopy to a wide variety of scientific problems. Highly competent technical staff provide all users access to routine as well as customized protocols involving fixation, embedding, sectioning and examination of samples. Pre- and Post-embed labeling procedures are available.

Core Facility Equipment

  • JEOL 1200 EXII Transmission Electron Microscope
  • JEOL 200 CX Transmission Electron Microscope

Publications Using the Core 

  • Shah AH, Rivas SR, Doucet-O'Hare TT, Govindarajan V, DeMarino C, Wang T, Ampie L, Zhang Y, Banasavadi-Siddegowda YK, Walbridge S, Maric D, Garcia-Montojo M, Suter RK, Lee MH, Zaghloul KA, Steiner J, Elkahloun AG, Chandar J, Seetharam D, Desgraves J, Li W, Johnson K, Ivan ME, Komotar RJ, Gilbert MR, Heiss JD, Nath A. Human endogenous retrovirus K contributes to a stem cell niche in glioblastoma. J Clin Invest. 2023; 133: e167929.
  • Shammas MK, Huang X, Wu BP, Fessler E, Song IY, Randolph NP, Li Y, Bleck CK, Springer DA, Fratter C, Barbosa IA, Powers AF, Quirs PM, Lopez-Otin C, Jae LT, Poulton J, Narendra DP. OMA1 mediates local and global stress responses against protein misfolding in CHCHD10 mitochondrial myopathy. J Clin Invest. 2022; 132: e157504.
  • Zhu PP, Hung HF, Batchenkova N, Nixon-Abell J, Henderson J, Zheng P, Renvois B, Pang S, Xu CS, Saalfeld S, Funke J, Xie Y, Svara F, Hess HF, Blackstone C. Transverse endoplasmic reticulum expansion in hereditary spastic paraplegia corticospinal axons. Hum Mol Genet. 2022: ddac072.
  • Liu, L., Dodd, S., Hunt, R., Pothayee, N., Atanasijevic, T., Bouraoud, N., Maric, D., Moseman, E., Gossa, S., McGavern, D., and Koretsky, A. Early detection of cerebrovascular pathology and protective antiviral immunity by MRI. eLife. 2022; 11: e74462.
  • Arpino, G., Somasundaram, A., Shin, W., Ge, L., Villareal, S., Chan, C.Y., Ashery, U., Shupliakov, O., Taraska, J.W. & Wu, L.G. Clathrin-mediated endocytosis cooperates with bulk endocytosis to generate vesicles. iScience 25, 103809 (2022).
  • Chamberlain KA, Huang N, Xie Y, LiCausi F, Li S, Li Y, Sheng ZH. Oligodendrocytes enhance axonal energy metabolism by deacetylation of mitochondrial proteins through transcellular delivery of SIRT2. Neuron. 2021: S0896-6273(21)00608-5.
  • Creighton BA, Afriyie S, Ajit D, Casingal CR, Voos KM, Reger J, Burch AM, Dyne E, Bay J, Huang JK, Anton ES, Fu MM, Lorenzo DN. Giant ankyrin-B mediates transduction of axon guidance and collateral branch pruning factor sema 3A. Elife. 2021; 10:e 69815. ​​​​​​​