Dr. Cheng received her B.S. from the Zoology Department of National Taiwan University in 1973 and her Ph.D. from Purdue University in 1978, where she studied the activity-dependent structural changes of a mechano-receptor at the electron microscopy level under the guidance of Dr. Yasuko Nakajima. She then did her postdoctoral research with Dr. Jack Rosenbluth at the New York University Medical School focusing on the development and disease-related structural changes of the node of Ranvier in mammalian central and peripheral nervous systems by EM. In 1984, she joined NINDS as a Fellow in Dr. Milton Brightman’s lab to study the in vitro interactions between brain endothelial cells and astrocytes, the two major components that form the blood-brain-barrier in vivo. In 1989, she became the Manager of the NINDS EM Facility. Dr. Cheng specializes in the pre-embedding immunogold labeling studies of various experimental systems, especially the ultrastructural details of synapses in dissociated and organotypic neuronal cultures, acute brain slices and perfusion-fixed brains.
The EM Facility provides intramural NINDS investigators with the opportunity to use electron microscopic techniques in their research programs. The Facility assists in all aspects of electron microscopy including project planning, specimen preparation and training.
- Assessment on whether EM is appropriate and feasible for user’s project
- Suggestion on optimal fixation methods
- Explanation on tissue processing time frame and what to expect
- Training users in using the TEM
- Assistance in interpretation of EM images