BG 10 RM 7C213
10 CENTER DR
BETHESDA MD 20814
Dr. Malik earned his undergraduate degree from Millersville University and his graduate degree from the Pennsylvania State University College of Medicine. His doctoral work involved identifying and characterizing the Na,K-ATPase b3 subunit. Upon completion of his doctoral studies he completed postdoctoral training at the National Institutes of Health in the laboratory of Dr. Heiner Westphal. The focus of his postdoctoral studies was mammalian brain development utilizing the mouse as an animal model.
Dr. Malik works in the NINDS Translational Neuroscience Center to generate stem cell lines that are used for a variety of drug screening assays to facilitate discovery of compounds that may have potency in the treatment of neurodegenerative disorders. He primarily works with human neural stem cells (NSCs) and takes advantage of an array of gene editing tools to knock-in, knock-out, or conditionally express a variety of proteins in NSCs. The edited NSCs serve as screening vehicles in both their multipotent stem cell state and in the differentiated state (neurons and glia).
Malik N, Manickam R, Bachani M, Steiner JP. (2019) A strategy to identify compounds that affect cell growth and survival in cultured mammalian cells at low-to-moderate throughput. J Vis Exp 151.
Francis KR, Ton AN, Xin Y, O’Halloran PE, Wassif CA, Malik N, Williams IM, Cluzeau CV, Trivedi NS, Pavan WJ, Cho W, Westphal H, Porter FD. (2016) Modeling Smith-Lemli-Opitz syndrome with induced pluripotent stem cells reveals a causal role for Wnt/b-catenin defects in neuronal cholesterol synthesis phenotypes. Nat Med: 22: 388-96.
Hunsberger J, Efthymiou AG, Malik N, Behl M, Mead IL, Zeng X, Simeonov A, Rao M. (2015) Induced pluripotent stem cell models enable in vitro models for screening in the CNS. Stem Cells Dev: 2015 Mar 20. Epub ahead of print.
Efthymiou A, Steiner J, Pavan WJ, Wincovitch S, Larson DM, Porter FD, Rao MS, Malik N (2015) Rescue of an in vitro neuron phenotype identified using Niemann Pick-C iPSC line derived neurons by modulating the WNT pathway and calcium signaling. Stem Cells Transl Med 4: 230-238.
Cerbini T, Funahashi R, Luo Y, Liu C, Park K-Y, Heman-Ackah S, Rao M, Malik N, Zou J (2015) TALEN-mediated CLYBL targeting enables enhanced transgene expression and one-step generation of multiplexed reporter human iPSC and NSC lines. Manuscript accepted PLoS One 10: e0116032.
Malik N, Efthymiou AG, Mather K, Chester N, Wang X, Nath A, Rao MS, Steiner JP (2014) Compounds with species and cell type specific toxicity identified in a 2000 compound drug screen of neural stem cells and rat mixed cortical neurons. Neurotoxicology 45C: 192-200.