Dragan Maric, Ph.D.

Headshot of Dragan Maric, PHD

Dragan Maric, Ph.D.

Core Director and Staff Scientist
Address
FLOW AND IMAGING CYTOMETRY CORE FACILITY

BG 10 RM 5S241
10 CENTER DR
BETHESDA MD 20814

Dr. Dragan Maric received B.S. degrees in biochemistry and microbiology in 1981 from University of Maryland, and a M.S. in immunology in 1985 and a Ph.D. in neuroimmunology in 1989 from University of Belgrade, Yugoslavia. Dr. Maric completed his postdoctoral training in developmental neurobiology at NINDS from 1991-1993 and in mucosal immunology at McMaster University, Hamilton, Canada from 1993-1994. After returning to NINDS in 1994, Dr. Maric worked in the Laboratory of Neurophysiology first as a visiting scientist from 1994-2000 and then as a staff scientist from 2000-2010. He became Manager of the NINDS Flow and Imaging Cytometry Core Facility in 2001.

Dr. Maric’s primary interests include: researching the changing seminal properties of neural stem cells (NSCs) and their differentiating progeny during embryonic, postnatal and adult CNS development and adapting new methodologies to more effectively study the complex systems biology in CNS tissues under physiological and pathophysiological conditions. Dr. Maric is continuously developing novel multiplex fluorescence flow cytometry and in situ cytometry imaging bioassays aimed toward elucidating the underlying molecular and cellular mechanisms of NSC fate determination and regenerative potential during normal CNS development and in specific neuropathological and neurodegenerative disorders.

These studies also involve the longitudinal assessment of in vivo survival, differentiation and integration of FACS-sorted embryonic NSCs after implantation of these cells into the rodent brains following ischemic and traumatic brain injuries. He is also developing state-of-the-art multiplex fluorescence immunohistochemistry biomarker screening methods utilizing unique combinations up to 100 biomarkers relevant to comprehensively study the complex processes brain tissue remodeling and recovery in response to different types of insults (including rodent models of stroke as illustrated in the image below) and other neurodegenerative disorders in the CNS.

 

The image shows complex tissue remodeling in the coronal section of a rat brain following focal ischemic brain injury
The image shows complex tissue remodeling in the coronal section of a rat brain following focal ischemic brain injury.

 

The section was simultaneously probed with 12 different fluorescent biomarkers relevant to neuroinflammation, neuronal plasticity, neurogenesis, gliogenesis and angiogenesis and each biomarker imaged in a separate fluorescence channel using multi-spectral wide field fluorescence microscopy. The images were then merged into a 12-color fluorescence composite to illustrate the concurrent processes of brain tissue damage and repair in response to focal ischemia. The above methodology exemplifies a comprehensive multiplex biomarker screening approach to study complex systems biology in the brain under normal and pathological conditions assayed at single cell level of spatial resolution.